abstract：:Previous studies on DNA repair in ageing have demonstrated increased frequencies of single and double strand breaks in lymphocytes from elderly subjects and, as a consequence, decreased efficiency in DNA replication. We have investigated the relationship between cell proliferation and the nuclear expression of ku prot...

journal_title：Mechanisms of ageing and development

authors： Frasca D,Barattini P,Goso C,Pucci S,Rizzo G,Bartoloni C,Costanzo M,Errani A,Guidi L,Antico L,Tricerri A,Doria G

更新日期：1998-01-30 00:00:00

abstract：:A nuclease that releases noncomplementary nucleotides from the 3'-end of DNA was isolated and highly purified from rat liver extract. The d(T9-C) priming activities for DNA synthesis in vitro by DNA polymerases alpha and beta were recovered by the addition of this enzyme, which itself does not contain a DNA polymerase...

journal_title：Mechanisms of ageing and development

authors： Taguchi T,Toda T,Fukuda M,Ohashi M

更新日期：1998-01-12 00:00:00

abstract：:The binding of the lectin Concanavalin-A (Con-A) to crystallins was investigated. For this purpose, human fetal and juvenile lens crystallins were isofocused and specifically stained brown for glycoproteins by the lectin Con-A, and purple by the periodic acid Schiff's reagent (PAS). In reference experiments protein ba...

journal_title：Mechanisms of ageing and development

authors： Ahrend MH,Bours J

更新日期：1997-12-30 00:00:00

abstract：:Nearly 40 years ago it was proposed that accumulation of mutations or increased levels of DNA damage might contribute to aging processes. Despite several correlative studies in this area, the answer as to whether genomic integrity contributes to aging has remained illusive. More recently it has been hypothesized that ...

journal_title：Mechanisms of ageing and development

authors： Walter CA,Grabowski DT,Street KA,Conrad CC,Richardson A

更新日期：1997-12-01 00:00:00

abstract：:In this paper we investigate nucleic acid content in neurons from the dorsocaudal region of the thalamic reticular nucleus in ageing Wistar rats. Nucleic acid per surface unit was analysed by calculating mean extinction using cytophotometric methods. Once the mean extinction and nuclear and cytoplasmic areas were know...

journal_title：Mechanisms of ageing and development

authors： Requena V,González F,Díaz F,Villena A,Peláez A,Pérez de Vargas I

更新日期：1997-12-01 00:00:00

abstract：:Glucocorticoids promote the development of many organs including intestine. At the cellular level, the activity of glucocorticoids is regulated by 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) which converts active glucocorticoids to inactive metabolites. As 11 beta HSD is also expressed in the intestine, this en...

journal_title：Mechanisms of ageing and development

authors： Pohlová I,Miksík I,Pácha J

更新日期：1997-11-01 00:00:00

abstract：:In a cell culture model of aging we have previously shown that there is an age-related decrease in the lipid dynamics of the proerythropoetic K562 cell membranes, as determined by the generalized polarization (GP) of the phase-sensitive lipid probe 2-dimethylamino-6-lauroylnaphthalene (Laurdan) (T. Parasassi, M. Di St...

journal_title：Mechanisms of ageing and development

authors： Levi M,Wilson P,Nguyen S,Iorio E,Sapora O,Parasassi T

更新日期：1997-08-01 00:00:00

abstract：:In a longitudinal design in which each animal was examined repeatedly throughout its life, we used flow cytometry to determine the numbers or proportions of a variety of T lymphocyte subsets in the peripheral blood of mice aged 8-20 months. Half of the 128 mice were given a low calorie diet which is known to extend li...

journal_title：Mechanisms of ageing and development

authors： Miller RA

更新日期：1997-06-01 00:00:00

abstract：:The objective of the this study was to determine the cytokine profile of aging mice and to establish whether changes in cytokine production account for the fact that aging mice develop a milder disease than the young in response to induced experimental systemic lupus erythematosus (SLE). Cytokine secretion was evaluat...

journal_title：Mechanisms of ageing and development

authors： Segal R,Dayan M,Globerson A,Habut B,Shearer GM,Mozes E

更新日期：1997-06-01 00:00:00

abstract：:The present study was designed to determine whether the memory sparing effects of dietary restriction during aging could be through an effect on ionotropic glutamate receptors. Quantitative autoradiography was performed on 3, 10, and 26 month old mice to examine the density changes of NMDA, AMPA and kainate binding si...

journal_title：Mechanisms of ageing and development

authors： Magnusson KR

更新日期：1997-05-01 00:00:00

abstract：:Age-related and ambient temperature-related changes in motor activity, body temperature, body weight (b.w.), and food consumption were studied in the long-lived Peromyscus leucopus mouse at environmental temperatures of 29 and 21 degrees C. Major changes in physiological performance were observed between the young (6 ...

journal_title：Mechanisms of ageing and development

authors： Duffy PH,Feuers RJ,Pipkin JL,Turturro A,Hart RW

更新日期：1997-04-01 00:00:00

abstract：:In the present study we have investigated the prevalence of organ-specific and non organ-specific autoantibodies in 26 healthy centenarians (6 men, 20 women; age range 101-106 years), using as controls 54 healthy old (33 men and 21 women, age range 71-93) and 56 young subjects (29 men and 27 women, age range 26-60). W...

journal_title：Mechanisms of ageing and development

authors： Candore G,Di Lorenzo G,Mansueto P,Melluso M,Fradà G,Li Vecchi M,Esposito Pellitteri M,Drago A,Di Salvo A,Caruso C

更新日期：1997-03-01 00:00:00

abstract：:The decline in the lymphoproliferative response to mitogenic stimuli shows marked heterogeneity in elderly individuals. Adequate nutriture is required for optimal immune function, yet nutritional status may be compromised in the elderly. To address whether this variation in the proliferative response of elderly indivi...

journal_title：Mechanisms of ageing and development

authors： Gardner EM,Bernstein ED,Dorfman M,Abrutyn E,Murasko DM

更新日期：1997-03-01 00:00:00

abstract：:We investigated the production of IL-2 and IFN-gamma (Th1 type) and IL-4 (Th2 type) cytokines by mitogen-activated spleen cells from young, adult and old mice. Cytokine production was evaluated in culture supernatants by CTLL proliferation (IL-2), ELISA (IFN-gamma), CT4.S proliferation (IL-4) and in mRNA extracted fro...

journal_title：Mechanisms of ageing and development

authors： Frasca D,Pucci S,Goso C,Barattini P,Barile S,Pioli C,Doria G

更新日期：1997-02-01 00:00:00

abstract：:Functional defects that accumulate in the T cell compartment are thought to be responsible for the pronounced immunodeficiency that develops during ageing, and reduced production of T cells by the thymus as it undergoes involution has been suggested to contribute to this phenomenon. Understanding the mechanisms respon...

journal_title：Mechanisms of ageing and development

authors： Montecino-Rodriguez E,Dorshkind K

更新日期：1997-02-01 00:00:00

abstract：:Motor neurons and ventral root motor fibres innervating extensor digitorum longus (EDL) muscle in rats of 0.5-3.5 months of age were studied by HRP-retrograde labelling and in vivo muscle tension recording, respectively. EDL nucleus size increased with age but motor neurons number and size did not change. Twitch and t...

journal_title：Mechanisms of ageing and development

authors： Cuppini R,Ambrogini P

更新日期：1996-12-20 00:00:00

abstract：:Quantitative interference microscopy was used to determine changes in nuclear and nucleolar indices (dry mass and cross-sectional area) in upper and lower epidermal cells and adjacent leaf-margin hair cells of the May apple (Podophyllum peltatum L.) leaves over a 42-day period (after leaves emerged above the ground li...

journal_title：Mechanisms of ageing and development

authors： Bhattacharya PK,Pappelis AJ,Lee SC,BeMiller JN,Karagiannis CS

更新日期：1996-12-20 00:00:00

abstract：:The age-related deficit in the dose response of osteoprogenitor cells to IGF-I was further investigated. As expected, the effective dose, but not the maximal effect, was shifted two orders of magnitude higher in old cells. In this paper, we examined whether this age-deficit can be attributed to an alteration in the ex...

journal_title：Mechanisms of ageing and development

authors： Tanaka H,Liang CT

更新日期：1996-11-29 00:00:00

abstract：:Ascorbic acid content decreased with age in livers of male rats but not in those of female rats. The influence of aging on the regeneration of ascorbic acid from dehydroascorbic acid was investigated as a possible cause of declining ascorbate concentrations. The results demonstrate that hepatic NADPH-dehydroascorbic a...

journal_title：Mechanisms of ageing and development

authors： Rikans LE,Lopez TR,Hornbrook KR

更新日期：1996-11-13 00:00:00

abstract：:The healing of normal incisional wounds and ischemic flap wounds was investigated in young (10 weeks) and old (102-104 weeks) rats, together with the effect of treatment with hyperbaric oxygen on day 0-3 of healing. After 10 days of healing all biomechanical strength parameters of normal wounds were decreased by 30-40...

journal_title：Mechanisms of ageing and development

authors： Quirinia A,Viidik A

更新日期：1996-10-25 00:00:00

abstract：:The aim of the present study was to measure protein synthesis in regenerating liver and to evaluate the impact of malnutrition in young and old rats. Two groups of male Wistar rats were used: young rats (4 months old) and old rats (18 months old). The rats were allocated to malnutrition or ordinary food intake for 1 w...

journal_title：Mechanisms of ageing and development

authors： Carrillo MC,Carnovale CE,Favre C,Monti JA,Scapini C

更新日期：1996-10-04 00:00:00

abstract：:Atypical bodies (ABs), related to aging, are described in the central nervous system of normal aged hamsters. Our study used ultrastructural microscopy and quantitative stereology analysis to study these structures in the hypothalamus and brain stem of 3, 6, 12, 18, 24 and 30 month-old hamsters. We found that these co...

journal_title：Mechanisms of ageing and development

authors： Navarro A,González del Rey C,Tolivia J,Alvarez-Uría M

更新日期：1996-09-09 00:00:00

abstract：:The impairment of brown adipose tissue (BAT) thermogenic activity with aging has been well documented. The current study investigated the effect of swimming training on BAT activity in 2-month-old (young) and 26-month-old (old) male mice. The trained mice underwent a 6-week swimming program (1 h/day, 5 days/week) in w...

journal_title：Mechanisms of ageing and development

authors： Oh-ishi S,Kizaki T,Toshinai K,Haga S,Fukuda K,Nagata N,Ohno H

更新日期：1996-08-15 00:00:00

abstract：:Age-specific mortality rates from breast cancer (BC) in Japan from 1950 through 1993 were subjected to longitudinal Gompertzian and Weibull analyses. Age-specific BC mortality rate distributions between the ages of 30 and 75 years were Gompertzian in each year. Between 1950 and 1993, age-adjusted BC mortality rates in...

journal_title：Mechanisms of ageing and development

authors： Imaizumi Y

更新日期：1996-07-17 00:00:00

abstract：:The purpose of these studies was to analyze the role of different immune cell populations in the immune response against Trypanosoma cruzi antigens in aged mice. Mice of different ages (3 and 12 months old) were immunized i.d. with S-105 plus Bordetella pertussis as adjuvant and we compared the activities of the lymph...

journal_title：Mechanisms of ageing and development

authors： Maletto BA,Gruppi A,Moron G,Pistoresi-Palencia MC

更新日期：1996-07-05 00:00:00

abstract：:Falls following a meal occur commonly in older persons. These falls have been related to a decrease in postprandial blood pressure due to carbohydrates in the meal. The mechanism by which this occurs is not known. In this study, we examined the possible role of a vasodilatory peptide, calcitonin gene-related peptide (...

journal_title：Mechanisms of ageing and development

authors： Edwards BJ,Perry HM 3rd,Kaiser FE,Morley JE,Kraenzle D,Stevenson R,Kreutter D

更新日期：1996-06-07 00:00:00

abstract：:The proto-oncogene c-fos (the cellular homolog of v-fos, Finkel-Biskis-Jenkins (FBJ) murine osteogenic sarcoma virus) encodes a major component of the activator protein-1 (AP-1) transcription factor. Serum stimulation as well as oxidizing treatments induce transitory increases in c-fos mRNA abundance. The induction of...

journal_title：Mechanisms of ageing and development

authors： Keogh BP,Tresini M,Cristofalo VJ,Allen RG

更新日期：1996-03-29 00:00:00

abstract：:Rats from one month to 22 months of age were subjected to in vivo brain microdialysis under a free-moving condition. The 3,4-dihydroxyphenylacetic acid level in the striatum reached a maximum at 3 months old. However, both basal and potassium-induced dopamine release were maximal at 1.5 months, which represents sexual...

journal_title：Mechanisms of ageing and development

authors： Nakano M,Mizuno T

更新日期：1996-02-24 00:00:00

abstract：:Age-specific mortality rates from stomach cancer (SC) in Japan from 1950 through 1993 were subjected to longitudinal Gompertzian analysis. Age-specific SC mortality rate distributions between ages 45 and 75 years were highly Gompertzian for each- and every year for both men and women. Between 1950 and 1993, age-adjust...

journal_title：Mechanisms of ageing and development

authors： Imaizumi Y

更新日期：1995-11-24 00:00:00

abstract：:In the rat, kininogens are produced from two distinctly regulated gene families, T- and K-kininogens. We have previously established that T-kininogen (T-KG, thiostatin) gene expression is increased in the liver of old rats, as compared to their younger counterparts. In this report, we have used Northern blot analysis ...

journal_title：Mechanisms of ageing and development

authors： Sierra F

更新日期：1995-10-13 00:00:00

abstract：:Age-specific mortality from carcinoma of the prostate was analyzed by drawing a line between the value at age 52 years and that at age 87. Slope of the line was relatively constant for the pooled years 1962-1966, 1967-1971 and 1972-1976 (maximum variation from the mean of 4.5%). However, the slope was 17.7% greater fo...

journal_title：Mechanisms of ageing and development

authors： Clive JM,Spencer RP

更新日期：1995-08-31 00:00:00

abstract：:Human diploid cells, TIG-7, were serially cultivated under 1%, 5% or 21% (air) oxygen. The limit of their cumulative population doublings was extended by 16% or 10% under 1% or 5% oxygen, respectively, as compared with that under 21% oxygen. When TIG-7 cells were serially cultivated in the presence of 10 mM or 20 mM 3...

journal_title：Mechanisms of ageing and development

authors： Yuan H,Kaneko T,Matsuo M

更新日期：1995-07-14 00:00:00

abstract：:Nitric oxide synthase (NOS) activity was enhanced in human umbilical vein endothelial cells (HUVECs) by the combined stimulation with IFN-gamma plus IL-1beta, TNF-alpha and LPS which was accompanied by cell death. DNA analysis of the NOS induced dead HUVECs showed that internucleosomal DNA fragmentation had occurred, ...

journal_title：Mechanisms of ageing and development

authors： Sato I,Kaji K,Murota S

更新日期：1995-06-30 00:00:00

abstract：:Lipofuscin accumulation in cultured rat myocardial cells is considered an index of intra-lysosomal oxidative reactions and was registered by autofluorescence measurements. Lipofuscinogenesis in secondary lysosomes is thought to be a consequence of Fenton reactions, and is much enhanced by oxidative stress obtained by ...

journal_title：Mechanisms of ageing and development

authors： Marzabadi MR,Jones C,Rydström J

更新日期：1995-06-09 00:00:00

abstract：:Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-m...

journal_title：Mechanisms of ageing and development

authors： Bosman GJ,Steetzel BD,De Man AJ,Van Kalmthout PJ,Visser FE,De Grip WJ

更新日期：1995-04-21 00:00:00

abstract：:We investigated the Coenzyme Q (CoQ) content of different mitochondrial fractions [free mitochondria (FM), synaptic heavy (HM) and light mitochondria (LM)] from three brain areas (cortex, striatum, hippocampus) of rats at different ages. In rats from 2 to 26 months of age, we observed only small differences in total C...

journal_title：Mechanisms of ageing and development

authors： Battino M,Gorini A,Villa RF,Genova ML,Bovina C,Sassi S,Littarru GP,Lenaz G

更新日期：1995-03-17 00:00:00

abstract：:Glutathione transferases (GSTs; EC. 2.1.5.18) activity was measured in maternal liver and conceptal tissues during gestation. In maternal liver, maximum activity was found at gestational day (GD) 9 after which it slowly decreased up to the end of gestation. The placental GSTs activity at GD18 was three times lower tha...

journal_title：Mechanisms of ageing and development

authors： Di Ilio C,Tiboni GM,Sacchetta P,Angelucci S,Bucciarelli T,Bellati U,Aceto A

更新日期：1995-01-31 00:00:00

abstract：:As a prerequisite to investigating the specification and differentiation of cardiac tissue in vitro, the ontogeny of a number of putative cardiac-specific, and striated muscle-specific gene transcripts has been studied. The probes used include cDNAs of alpha-actins, myosin heavy chains, myosin light chains, alpha-trop...

journal_title：Mechanisms of ageing and development

authors： Murrell WG,Masters CJ,Willis RJ,Crane DI

更新日期：1994-12-16 00:00:00

abstract：:Previous studies from this laboratory have documented a progressive age-related decline in trophic hormone (or second messenger cAMP) stimulated corticosterone production in isolated adrenocortical cells. In the current study, we examined the possibility that the aging process exerts this effect by interfering with an...

journal_title：Mechanisms of ageing and development

authors： Azhar S,Reaven E

更新日期：1994-11-25 00:00:00

abstract：:Different theories of ageing exist. Oxidative stress by generating neo-antigens and affecting cellular communications may precipitate immune disregulation, and both may concur in the whole ageing process. In the present study, we have analysed the effect of dietary high doses of vitamin E, a free radical scavenger, on...

journal_title：Mechanisms of ageing and development

authors： Brohée D,Nève P

更新日期：1994-10-20 00:00:00